Speaker
Description
Abstract
Preclinical studies often overlook lung mucus barriers, crucial for drug permeation. This research uses a patient-derived 3D cell culture model to examine lanthanide-doped upconverting nanoparticles (UCNPs) with different surface coatings. Tracking UCNPs via upconverting emissions reveals significant differences in mucus residence time and mucociliary clearance. Positively charged, ligand-free, and negatively charged, supported lipid bilayer-coated UCNPs exhibit distinct behaviors. These findings emphasize the importance of considering nanoparticle surface properties in preclinical investigations, particularly in tissue models, highlighting the need for realistic assessments to improve therapeutic outcomes.
1 Result and Discussion
Lipid bilayered upconversion nanoparticle permeating mucus layer
When nanoparticles were aerosol-deposed on the mucosa epithelium, SLB- and LF-UCNPs were homogeneously distributed over the mucus layer and by the mucociliary movement, this deposition is mostly cleared out from the center of the MucilAir. Figure 1 shows the images at the center of MucilAir inserts measured 24 hours after aerosol deposition for the two UCNPs preparations. SLB-UCNPs are detected over the epithelial tissue layer. We investigated the cellular interaction of SLB-UCNPs after avoiding the interaction with mucus. LF-UCNPs were not detected on the mucus layer and were fully cleared out by mucociliary clearance after 24 hours.
2 Conclusion
This study underscores the efficacy of lipid-coated upconverting nanoparticles (UCNPs) for mucus permeation in realistic aerosol delivery, offering insights for personalized medical solutions and broader applications in treating various mammalian tissues.
